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    Oxford Instruments trackingtoolpro gradientech gradientech se matlab open source platform
    Trackingtoolpro Gradientech Gradientech Se Matlab Open Source Platform, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44473 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trackingtoolpro+gradientech+gradientech+se+matlab+open+source+platform/Imaris/pm40712579-394-186-193
    Average 99 stars, based on 44473 article reviews
    trackingtoolpro gradientech gradientech se matlab open source platform - by Bioz Stars, 2026-10
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    Expressing:

    Article Title: Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MLC T18S19 antibody Cell Signaling Cat# 95777S; RRID: AB_3677547 PE Anti-Mouse Ly6G (Gr1) antibody Biolegend Cat# 127608; RRID: AB_1186099 Anti-GFP antibody Roche Cat# 11814460001; RRID: AB_390913 Anti-RFP antibody TaKaRa Cat# 632496; RRID: AB_10013483 Anti-β-actin antibody Zen Bioscience Cat# 200068-8F10; RRID: AB_2722710 Bacterial and virus strains mCherry-His expressing E. coli strain Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Klebsiella aerogenes Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Chemicals, peptides, and recombinant proteins T4 DNA Ligase NEB Cat# M0202V G418 Thermo Fisher Scientific Cat# 10131035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Blasticidin S InvivoGen Cat# ant-bl-10p Na 2 HPO 4 Sangon Biotech Cat# 7558-79-4 NaH 2 PO 4 Sangon Biotech Cat# 7558-80-7 NaCl Sangon Biotech Cat# 7647-14-5 KCl Sangon Biotech Cat# 7447-40-7 CaCl 2 Sangon Biotech Cat# 10043-52-4 Poly-lysine Sigma Cat# P4707-50mL Sir-actin Cytoskeleton Cat# CY-SC001 Proteinase K TaKaRa Cat# 9034 RNase A TaKaRa Cat# 2158-1 APTES Sigma Cat# A3648-100mL Cell-tak Corning Cat# 354240 EGTA Sigma Cat# 324626 Blebbistatin Selleck Cat# E1249 UNC3230 Selleck Cat# E0305 ISA-2011B Selleck Cat# E1388 D-Sorbitol Sigma Cat# S7547-250G Formaldehyde NOVON Cat# SS2097 Glutaraldehyde Sigma Cat# G7651 Critical commercial assays PrimeSTAR GXL DNA Polymerase TaKaRa Cat# R050A 2 x Taq Master Mix Vazyme Cat# P112 TIANprep Mini Plasmid Kit TIANGEN Cat# DP103-02 PrimeScript RT reagent kit TaKaRa Cat# RR047A EasySep protocol Stemcell Technologies Cat# 19762A gDNA Eraser TaKaRa Cat# RR047B Deposited data Raw western blot data Mendeley Data https://doi.org/10.17632/fytvkm7v9d.1 Experimental models: Cell lines Dictyostelium discoideum AX2 cell line Robert Kay laboratory (MRC Laboratory of Molecular Biology, UK) N/A (Continued on next page) Developmental Cell 60, 1–16.e1–e5, December 15, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dictyostelium discoideum AX3 cell line Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum mhcA - cell line Douglas N. Robinson laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum racE - cell line Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Dictyostelium discoideum pikI - cell line This paper N/A Primary Neutrophils This paper N/A Oligonucleotides Primers for generation of expression constructs This paper Summarized in Table S1 Primers for generation of knockout cells This paper Summarized in Table S1 Recombinant DNA pDM323-myr-GFP This paper N/A pDM451-myr-RFP This paper N/A pCV5-GCaMP6f-mCherry This paper N/A pDM323-LifeAct-GFP This paper N/A pDM451-LifeAct-RFP This paper N/A pDM1285-pikI This paper N/A pDM451-RFP-pikI This paper N/A pDM449-RFP-cortexillin I This paper N/A pBig-GFP-mhcA Tom Egelhoff laboratory (Case Western Reserve School of Medicine, USA) N/A pDM323-PH plcδ -GFP Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A psadA-GFP Rex Chisholm laboratory (Northwestern University, USA) N/A pDM317-GFP-RacE Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Software and algorithms Olympus FV31S-SW Olympus www.olympus-global.com Fiji imageJ Open-source platform imagej.net/software/fiji/ Nikon NIS viewer Nikon www.microscope.healthcare.nikon.com/zh_CN/ Prism 9.0 GraphPad Software www.graphpad.com TrackingToolPro Gradientech gradientech.se MATLAB Open-source platform www.mathworks.com/products/matlab.html Imaris Oxford Instruments imaris.oxinst.com Beckman CytExpert Beckman www.mybeckman.cn/flow-cytometry/ research-flow-cytometers/cytoflex/software Other 35mm glass-bottom dish cellvis Cat# D35-20-1.5-N HL5 medium Formedium Cat# HLF2 GLU03 Formedium Cat# GLU03 SM agar Formedium Cat# SMA0102 agarose Yeasen Cat# 10208ES60 RPMI 1640 Medium Thermo Fisher Scientific Cat# C22400500BT BSA Solarbio Cat# A8010 Histopaque-1077 Sigma Cat# 10771 Histopaque-1119 Sigma Cat# 11191 e2 Developmental Cell 60, 1–16.e1–e5, December 15, 2025 .. Dictyostelium cell culture WT cells were derived from either the AX2 or AX3 axenic strain of Dictyostelium discoideum provided by Huaqing Cai laboratory (Chinese Academy of Sciences, China). mhcA - (originally made by the Douglas N. Robinson laboratory at Johns Hopkins University) and racE - cell lines were provided by the Huaqing Cai laboratory (Institute of Biophysics Chinese Academy of Sciences, China).

    Construct:

    Article Title: Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MLC T18S19 antibody Cell Signaling Cat# 95777S; RRID: AB_3677547 PE Anti-Mouse Ly6G (Gr1) antibody Biolegend Cat# 127608; RRID: AB_1186099 Anti-GFP antibody Roche Cat# 11814460001; RRID: AB_390913 Anti-RFP antibody TaKaRa Cat# 632496; RRID: AB_10013483 Anti-β-actin antibody Zen Bioscience Cat# 200068-8F10; RRID: AB_2722710 Bacterial and virus strains mCherry-His expressing E. coli strain Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Klebsiella aerogenes Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Chemicals, peptides, and recombinant proteins T4 DNA Ligase NEB Cat# M0202V G418 Thermo Fisher Scientific Cat# 10131035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Blasticidin S InvivoGen Cat# ant-bl-10p Na 2 HPO 4 Sangon Biotech Cat# 7558-79-4 NaH 2 PO 4 Sangon Biotech Cat# 7558-80-7 NaCl Sangon Biotech Cat# 7647-14-5 KCl Sangon Biotech Cat# 7447-40-7 CaCl 2 Sangon Biotech Cat# 10043-52-4 Poly-lysine Sigma Cat# P4707-50mL Sir-actin Cytoskeleton Cat# CY-SC001 Proteinase K TaKaRa Cat# 9034 RNase A TaKaRa Cat# 2158-1 APTES Sigma Cat# A3648-100mL Cell-tak Corning Cat# 354240 EGTA Sigma Cat# 324626 Blebbistatin Selleck Cat# E1249 UNC3230 Selleck Cat# E0305 ISA-2011B Selleck Cat# E1388 D-Sorbitol Sigma Cat# S7547-250G Formaldehyde NOVON Cat# SS2097 Glutaraldehyde Sigma Cat# G7651 Critical commercial assays PrimeSTAR GXL DNA Polymerase TaKaRa Cat# R050A 2 x Taq Master Mix Vazyme Cat# P112 TIANprep Mini Plasmid Kit TIANGEN Cat# DP103-02 PrimeScript RT reagent kit TaKaRa Cat# RR047A EasySep protocol Stemcell Technologies Cat# 19762A gDNA Eraser TaKaRa Cat# RR047B Deposited data Raw western blot data Mendeley Data https://doi.org/10.17632/fytvkm7v9d.1 Experimental models: Cell lines Dictyostelium discoideum AX2 cell line Robert Kay laboratory (MRC Laboratory of Molecular Biology, UK) N/A (Continued on next page) Developmental Cell 60, 1–16.e1–e5, December 15, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dictyostelium discoideum AX3 cell line Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum mhcA - cell line Douglas N. Robinson laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum racE - cell line Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Dictyostelium discoideum pikI - cell line This paper N/A Primary Neutrophils This paper N/A Oligonucleotides Primers for generation of expression constructs This paper Summarized in Table S1 Primers for generation of knockout cells This paper Summarized in Table S1 Recombinant DNA pDM323-myr-GFP This paper N/A pDM451-myr-RFP This paper N/A pCV5-GCaMP6f-mCherry This paper N/A pDM323-LifeAct-GFP This paper N/A pDM451-LifeAct-RFP This paper N/A pDM1285-pikI This paper N/A pDM451-RFP-pikI This paper N/A pDM449-RFP-cortexillin I This paper N/A pBig-GFP-mhcA Tom Egelhoff laboratory (Case Western Reserve School of Medicine, USA) N/A pDM323-PH plcδ -GFP Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A psadA-GFP Rex Chisholm laboratory (Northwestern University, USA) N/A pDM317-GFP-RacE Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Software and algorithms Olympus FV31S-SW Olympus www.olympus-global.com Fiji imageJ Open-source platform imagej.net/software/fiji/ Nikon NIS viewer Nikon www.microscope.healthcare.nikon.com/zh_CN/ Prism 9.0 GraphPad Software www.graphpad.com TrackingToolPro Gradientech gradientech.se MATLAB Open-source platform www.mathworks.com/products/matlab.html Imaris Oxford Instruments imaris.oxinst.com Beckman CytExpert Beckman www.mybeckman.cn/flow-cytometry/ research-flow-cytometers/cytoflex/software Other 35mm glass-bottom dish cellvis Cat# D35-20-1.5-N HL5 medium Formedium Cat# HLF2 GLU03 Formedium Cat# GLU03 SM agar Formedium Cat# SMA0102 agarose Yeasen Cat# 10208ES60 RPMI 1640 Medium Thermo Fisher Scientific Cat# C22400500BT BSA Solarbio Cat# A8010 Histopaque-1077 Sigma Cat# 10771 Histopaque-1119 Sigma Cat# 11191 e2 Developmental Cell 60, 1–16.e1–e5, December 15, 2025 .. Dictyostelium cell culture WT cells were derived from either the AX2 or AX3 axenic strain of Dictyostelium discoideum provided by Huaqing Cai laboratory (Chinese Academy of Sciences, China). mhcA - (originally made by the Douglas N. Robinson laboratory at Johns Hopkins University) and racE - cell lines were provided by the Huaqing Cai laboratory (Institute of Biophysics Chinese Academy of Sciences, China).

    Knock-Out:

    Article Title: Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MLC T18S19 antibody Cell Signaling Cat# 95777S; RRID: AB_3677547 PE Anti-Mouse Ly6G (Gr1) antibody Biolegend Cat# 127608; RRID: AB_1186099 Anti-GFP antibody Roche Cat# 11814460001; RRID: AB_390913 Anti-RFP antibody TaKaRa Cat# 632496; RRID: AB_10013483 Anti-β-actin antibody Zen Bioscience Cat# 200068-8F10; RRID: AB_2722710 Bacterial and virus strains mCherry-His expressing E. coli strain Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Klebsiella aerogenes Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Chemicals, peptides, and recombinant proteins T4 DNA Ligase NEB Cat# M0202V G418 Thermo Fisher Scientific Cat# 10131035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Blasticidin S InvivoGen Cat# ant-bl-10p Na 2 HPO 4 Sangon Biotech Cat# 7558-79-4 NaH 2 PO 4 Sangon Biotech Cat# 7558-80-7 NaCl Sangon Biotech Cat# 7647-14-5 KCl Sangon Biotech Cat# 7447-40-7 CaCl 2 Sangon Biotech Cat# 10043-52-4 Poly-lysine Sigma Cat# P4707-50mL Sir-actin Cytoskeleton Cat# CY-SC001 Proteinase K TaKaRa Cat# 9034 RNase A TaKaRa Cat# 2158-1 APTES Sigma Cat# A3648-100mL Cell-tak Corning Cat# 354240 EGTA Sigma Cat# 324626 Blebbistatin Selleck Cat# E1249 UNC3230 Selleck Cat# E0305 ISA-2011B Selleck Cat# E1388 D-Sorbitol Sigma Cat# S7547-250G Formaldehyde NOVON Cat# SS2097 Glutaraldehyde Sigma Cat# G7651 Critical commercial assays PrimeSTAR GXL DNA Polymerase TaKaRa Cat# R050A 2 x Taq Master Mix Vazyme Cat# P112 TIANprep Mini Plasmid Kit TIANGEN Cat# DP103-02 PrimeScript RT reagent kit TaKaRa Cat# RR047A EasySep protocol Stemcell Technologies Cat# 19762A gDNA Eraser TaKaRa Cat# RR047B Deposited data Raw western blot data Mendeley Data https://doi.org/10.17632/fytvkm7v9d.1 Experimental models: Cell lines Dictyostelium discoideum AX2 cell line Robert Kay laboratory (MRC Laboratory of Molecular Biology, UK) N/A (Continued on next page) Developmental Cell 60, 1–16.e1–e5, December 15, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dictyostelium discoideum AX3 cell line Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum mhcA - cell line Douglas N. Robinson laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum racE - cell line Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Dictyostelium discoideum pikI - cell line This paper N/A Primary Neutrophils This paper N/A Oligonucleotides Primers for generation of expression constructs This paper Summarized in Table S1 Primers for generation of knockout cells This paper Summarized in Table S1 Recombinant DNA pDM323-myr-GFP This paper N/A pDM451-myr-RFP This paper N/A pCV5-GCaMP6f-mCherry This paper N/A pDM323-LifeAct-GFP This paper N/A pDM451-LifeAct-RFP This paper N/A pDM1285-pikI This paper N/A pDM451-RFP-pikI This paper N/A pDM449-RFP-cortexillin I This paper N/A pBig-GFP-mhcA Tom Egelhoff laboratory (Case Western Reserve School of Medicine, USA) N/A pDM323-PH plcδ -GFP Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A psadA-GFP Rex Chisholm laboratory (Northwestern University, USA) N/A pDM317-GFP-RacE Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Software and algorithms Olympus FV31S-SW Olympus www.olympus-global.com Fiji imageJ Open-source platform imagej.net/software/fiji/ Nikon NIS viewer Nikon www.microscope.healthcare.nikon.com/zh_CN/ Prism 9.0 GraphPad Software www.graphpad.com TrackingToolPro Gradientech gradientech.se MATLAB Open-source platform www.mathworks.com/products/matlab.html Imaris Oxford Instruments imaris.oxinst.com Beckman CytExpert Beckman www.mybeckman.cn/flow-cytometry/ research-flow-cytometers/cytoflex/software Other 35mm glass-bottom dish cellvis Cat# D35-20-1.5-N HL5 medium Formedium Cat# HLF2 GLU03 Formedium Cat# GLU03 SM agar Formedium Cat# SMA0102 agarose Yeasen Cat# 10208ES60 RPMI 1640 Medium Thermo Fisher Scientific Cat# C22400500BT BSA Solarbio Cat# A8010 Histopaque-1077 Sigma Cat# 10771 Histopaque-1119 Sigma Cat# 11191 e2 Developmental Cell 60, 1–16.e1–e5, December 15, 2025 .. Dictyostelium cell culture WT cells were derived from either the AX2 or AX3 axenic strain of Dictyostelium discoideum provided by Huaqing Cai laboratory (Chinese Academy of Sciences, China). mhcA - (originally made by the Douglas N. Robinson laboratory at Johns Hopkins University) and racE - cell lines were provided by the Huaqing Cai laboratory (Institute of Biophysics Chinese Academy of Sciences, China).

    Recombinant:

    Article Title: Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MLC T18S19 antibody Cell Signaling Cat# 95777S; RRID: AB_3677547 PE Anti-Mouse Ly6G (Gr1) antibody Biolegend Cat# 127608; RRID: AB_1186099 Anti-GFP antibody Roche Cat# 11814460001; RRID: AB_390913 Anti-RFP antibody TaKaRa Cat# 632496; RRID: AB_10013483 Anti-β-actin antibody Zen Bioscience Cat# 200068-8F10; RRID: AB_2722710 Bacterial and virus strains mCherry-His expressing E. coli strain Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Klebsiella aerogenes Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Chemicals, peptides, and recombinant proteins T4 DNA Ligase NEB Cat# M0202V G418 Thermo Fisher Scientific Cat# 10131035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Blasticidin S InvivoGen Cat# ant-bl-10p Na 2 HPO 4 Sangon Biotech Cat# 7558-79-4 NaH 2 PO 4 Sangon Biotech Cat# 7558-80-7 NaCl Sangon Biotech Cat# 7647-14-5 KCl Sangon Biotech Cat# 7447-40-7 CaCl 2 Sangon Biotech Cat# 10043-52-4 Poly-lysine Sigma Cat# P4707-50mL Sir-actin Cytoskeleton Cat# CY-SC001 Proteinase K TaKaRa Cat# 9034 RNase A TaKaRa Cat# 2158-1 APTES Sigma Cat# A3648-100mL Cell-tak Corning Cat# 354240 EGTA Sigma Cat# 324626 Blebbistatin Selleck Cat# E1249 UNC3230 Selleck Cat# E0305 ISA-2011B Selleck Cat# E1388 D-Sorbitol Sigma Cat# S7547-250G Formaldehyde NOVON Cat# SS2097 Glutaraldehyde Sigma Cat# G7651 Critical commercial assays PrimeSTAR GXL DNA Polymerase TaKaRa Cat# R050A 2 x Taq Master Mix Vazyme Cat# P112 TIANprep Mini Plasmid Kit TIANGEN Cat# DP103-02 PrimeScript RT reagent kit TaKaRa Cat# RR047A EasySep protocol Stemcell Technologies Cat# 19762A gDNA Eraser TaKaRa Cat# RR047B Deposited data Raw western blot data Mendeley Data https://doi.org/10.17632/fytvkm7v9d.1 Experimental models: Cell lines Dictyostelium discoideum AX2 cell line Robert Kay laboratory (MRC Laboratory of Molecular Biology, UK) N/A (Continued on next page) Developmental Cell 60, 1–16.e1–e5, December 15, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dictyostelium discoideum AX3 cell line Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum mhcA - cell line Douglas N. Robinson laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum racE - cell line Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Dictyostelium discoideum pikI - cell line This paper N/A Primary Neutrophils This paper N/A Oligonucleotides Primers for generation of expression constructs This paper Summarized in Table S1 Primers for generation of knockout cells This paper Summarized in Table S1 Recombinant DNA pDM323-myr-GFP This paper N/A pDM451-myr-RFP This paper N/A pCV5-GCaMP6f-mCherry This paper N/A pDM323-LifeAct-GFP This paper N/A pDM451-LifeAct-RFP This paper N/A pDM1285-pikI This paper N/A pDM451-RFP-pikI This paper N/A pDM449-RFP-cortexillin I This paper N/A pBig-GFP-mhcA Tom Egelhoff laboratory (Case Western Reserve School of Medicine, USA) N/A pDM323-PH plcδ -GFP Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A psadA-GFP Rex Chisholm laboratory (Northwestern University, USA) N/A pDM317-GFP-RacE Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Software and algorithms Olympus FV31S-SW Olympus www.olympus-global.com Fiji imageJ Open-source platform imagej.net/software/fiji/ Nikon NIS viewer Nikon www.microscope.healthcare.nikon.com/zh_CN/ Prism 9.0 GraphPad Software www.graphpad.com TrackingToolPro Gradientech gradientech.se MATLAB Open-source platform www.mathworks.com/products/matlab.html Imaris Oxford Instruments imaris.oxinst.com Beckman CytExpert Beckman www.mybeckman.cn/flow-cytometry/ research-flow-cytometers/cytoflex/software Other 35mm glass-bottom dish cellvis Cat# D35-20-1.5-N HL5 medium Formedium Cat# HLF2 GLU03 Formedium Cat# GLU03 SM agar Formedium Cat# SMA0102 agarose Yeasen Cat# 10208ES60 RPMI 1640 Medium Thermo Fisher Scientific Cat# C22400500BT BSA Solarbio Cat# A8010 Histopaque-1077 Sigma Cat# 10771 Histopaque-1119 Sigma Cat# 11191 e2 Developmental Cell 60, 1–16.e1–e5, December 15, 2025 .. Dictyostelium cell culture WT cells were derived from either the AX2 or AX3 axenic strain of Dictyostelium discoideum provided by Huaqing Cai laboratory (Chinese Academy of Sciences, China). mhcA - (originally made by the Douglas N. Robinson laboratory at Johns Hopkins University) and racE - cell lines were provided by the Huaqing Cai laboratory (Institute of Biophysics Chinese Academy of Sciences, China).

    Western Blot:

    Article Title: Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MLC T18S19 antibody Cell Signaling Cat# 95777S; RRID: AB_3677547 PE Anti-Mouse Ly6G (Gr1) antibody Biolegend Cat# 127608; RRID: AB_1186099 Anti-GFP antibody Roche Cat# 11814460001; RRID: AB_390913 Anti-RFP antibody TaKaRa Cat# 632496; RRID: AB_10013483 Anti-β-actin antibody Zen Bioscience Cat# 200068-8F10; RRID: AB_2722710 Bacterial and virus strains mCherry-His expressing E. coli strain Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Klebsiella aerogenes Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Chemicals, peptides, and recombinant proteins T4 DNA Ligase NEB Cat# M0202V G418 Thermo Fisher Scientific Cat# 10131035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Blasticidin S InvivoGen Cat# ant-bl-10p Na 2 HPO 4 Sangon Biotech Cat# 7558-79-4 NaH 2 PO 4 Sangon Biotech Cat# 7558-80-7 NaCl Sangon Biotech Cat# 7647-14-5 KCl Sangon Biotech Cat# 7447-40-7 CaCl 2 Sangon Biotech Cat# 10043-52-4 Poly-lysine Sigma Cat# P4707-50mL Sir-actin Cytoskeleton Cat# CY-SC001 Proteinase K TaKaRa Cat# 9034 RNase A TaKaRa Cat# 2158-1 APTES Sigma Cat# A3648-100mL Cell-tak Corning Cat# 354240 EGTA Sigma Cat# 324626 Blebbistatin Selleck Cat# E1249 UNC3230 Selleck Cat# E0305 ISA-2011B Selleck Cat# E1388 D-Sorbitol Sigma Cat# S7547-250G Formaldehyde NOVON Cat# SS2097 Glutaraldehyde Sigma Cat# G7651 Critical commercial assays PrimeSTAR GXL DNA Polymerase TaKaRa Cat# R050A 2 x Taq Master Mix Vazyme Cat# P112 TIANprep Mini Plasmid Kit TIANGEN Cat# DP103-02 PrimeScript RT reagent kit TaKaRa Cat# RR047A EasySep protocol Stemcell Technologies Cat# 19762A gDNA Eraser TaKaRa Cat# RR047B Deposited data Raw western blot data Mendeley Data https://doi.org/10.17632/fytvkm7v9d.1 Experimental models: Cell lines Dictyostelium discoideum AX2 cell line Robert Kay laboratory (MRC Laboratory of Molecular Biology, UK) N/A (Continued on next page) Developmental Cell 60, 1–16.e1–e5, December 15, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dictyostelium discoideum AX3 cell line Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum mhcA - cell line Douglas N. Robinson laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum racE - cell line Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Dictyostelium discoideum pikI - cell line This paper N/A Primary Neutrophils This paper N/A Oligonucleotides Primers for generation of expression constructs This paper Summarized in Table S1 Primers for generation of knockout cells This paper Summarized in Table S1 Recombinant DNA pDM323-myr-GFP This paper N/A pDM451-myr-RFP This paper N/A pCV5-GCaMP6f-mCherry This paper N/A pDM323-LifeAct-GFP This paper N/A pDM451-LifeAct-RFP This paper N/A pDM1285-pikI This paper N/A pDM451-RFP-pikI This paper N/A pDM449-RFP-cortexillin I This paper N/A pBig-GFP-mhcA Tom Egelhoff laboratory (Case Western Reserve School of Medicine, USA) N/A pDM323-PH plcδ -GFP Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A psadA-GFP Rex Chisholm laboratory (Northwestern University, USA) N/A pDM317-GFP-RacE Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Software and algorithms Olympus FV31S-SW Olympus www.olympus-global.com Fiji imageJ Open-source platform imagej.net/software/fiji/ Nikon NIS viewer Nikon www.microscope.healthcare.nikon.com/zh_CN/ Prism 9.0 GraphPad Software www.graphpad.com TrackingToolPro Gradientech gradientech.se MATLAB Open-source platform www.mathworks.com/products/matlab.html Imaris Oxford Instruments imaris.oxinst.com Beckman CytExpert Beckman www.mybeckman.cn/flow-cytometry/ research-flow-cytometers/cytoflex/software Other 35mm glass-bottom dish cellvis Cat# D35-20-1.5-N HL5 medium Formedium Cat# HLF2 GLU03 Formedium Cat# GLU03 SM agar Formedium Cat# SMA0102 agarose Yeasen Cat# 10208ES60 RPMI 1640 Medium Thermo Fisher Scientific Cat# C22400500BT BSA Solarbio Cat# A8010 Histopaque-1077 Sigma Cat# 10771 Histopaque-1119 Sigma Cat# 11191 e2 Developmental Cell 60, 1–16.e1–e5, December 15, 2025 .. Dictyostelium cell culture WT cells were derived from either the AX2 or AX3 axenic strain of Dictyostelium discoideum provided by Huaqing Cai laboratory (Chinese Academy of Sciences, China). mhcA - (originally made by the Douglas N. Robinson laboratory at Johns Hopkins University) and racE - cell lines were provided by the Huaqing Cai laboratory (Institute of Biophysics Chinese Academy of Sciences, China).

    Software:

    Article Title: Inchworm migration facilitates amoeboid cell adaptation to high-adhesion environments.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MLC T18S19 antibody Cell Signaling Cat# 95777S; RRID: AB_3677547 PE Anti-Mouse Ly6G (Gr1) antibody Biolegend Cat# 127608; RRID: AB_1186099 Anti-GFP antibody Roche Cat# 11814460001; RRID: AB_390913 Anti-RFP antibody TaKaRa Cat# 632496; RRID: AB_10013483 Anti-β-actin antibody Zen Bioscience Cat# 200068-8F10; RRID: AB_2722710 Bacterial and virus strains mCherry-His expressing E. coli strain Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Klebsiella aerogenes Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Chemicals, peptides, and recombinant proteins T4 DNA Ligase NEB Cat# M0202V G418 Thermo Fisher Scientific Cat# 10131035 Hygromycin B Thermo Fisher Scientific Cat# 10687010 Blasticidin S InvivoGen Cat# ant-bl-10p Na 2 HPO 4 Sangon Biotech Cat# 7558-79-4 NaH 2 PO 4 Sangon Biotech Cat# 7558-80-7 NaCl Sangon Biotech Cat# 7647-14-5 KCl Sangon Biotech Cat# 7447-40-7 CaCl 2 Sangon Biotech Cat# 10043-52-4 Poly-lysine Sigma Cat# P4707-50mL Sir-actin Cytoskeleton Cat# CY-SC001 Proteinase K TaKaRa Cat# 9034 RNase A TaKaRa Cat# 2158-1 APTES Sigma Cat# A3648-100mL Cell-tak Corning Cat# 354240 EGTA Sigma Cat# 324626 Blebbistatin Selleck Cat# E1249 UNC3230 Selleck Cat# E0305 ISA-2011B Selleck Cat# E1388 D-Sorbitol Sigma Cat# S7547-250G Formaldehyde NOVON Cat# SS2097 Glutaraldehyde Sigma Cat# G7651 Critical commercial assays PrimeSTAR GXL DNA Polymerase TaKaRa Cat# R050A 2 x Taq Master Mix Vazyme Cat# P112 TIANprep Mini Plasmid Kit TIANGEN Cat# DP103-02 PrimeScript RT reagent kit TaKaRa Cat# RR047A EasySep protocol Stemcell Technologies Cat# 19762A gDNA Eraser TaKaRa Cat# RR047B Deposited data Raw western blot data Mendeley Data https://doi.org/10.17632/fytvkm7v9d.1 Experimental models: Cell lines Dictyostelium discoideum AX2 cell line Robert Kay laboratory (MRC Laboratory of Molecular Biology, UK) N/A (Continued on next page) Developmental Cell 60, 1–16.e1–e5, December 15, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dictyostelium discoideum AX3 cell line Peter Devreotes laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum mhcA - cell line Douglas N. Robinson laboratory (Johns Hopkins University, USA) N/A Dictyostelium discoideum racE - cell line Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Dictyostelium discoideum pikI - cell line This paper N/A Primary Neutrophils This paper N/A Oligonucleotides Primers for generation of expression constructs This paper Summarized in Table S1 Primers for generation of knockout cells This paper Summarized in Table S1 Recombinant DNA pDM323-myr-GFP This paper N/A pDM451-myr-RFP This paper N/A pCV5-GCaMP6f-mCherry This paper N/A pDM323-LifeAct-GFP This paper N/A pDM451-LifeAct-RFP This paper N/A pDM1285-pikI This paper N/A pDM451-RFP-pikI This paper N/A pDM449-RFP-cortexillin I This paper N/A pBig-GFP-mhcA Tom Egelhoff laboratory (Case Western Reserve School of Medicine, USA) N/A pDM323-PH plcδ -GFP Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A psadA-GFP Rex Chisholm laboratory (Northwestern University, USA) N/A pDM317-GFP-RacE Huaqing cai laboratory (Chinese Academy of Sciences, China) N/A Software and algorithms Olympus FV31S-SW Olympus www.olympus-global.com Fiji imageJ Open-source platform imagej.net/software/fiji/ Nikon NIS viewer Nikon www.microscope.healthcare.nikon.com/zh_CN/ Prism 9.0 GraphPad Software www.graphpad.com TrackingToolPro Gradientech gradientech.se MATLAB Open-source platform www.mathworks.com/products/matlab.html Imaris Oxford Instruments imaris.oxinst.com Beckman CytExpert Beckman www.mybeckman.cn/flow-cytometry/ research-flow-cytometers/cytoflex/software Other 35mm glass-bottom dish cellvis Cat# D35-20-1.5-N HL5 medium Formedium Cat# HLF2 GLU03 Formedium Cat# GLU03 SM agar Formedium Cat# SMA0102 agarose Yeasen Cat# 10208ES60 RPMI 1640 Medium Thermo Fisher Scientific Cat# C22400500BT BSA Solarbio Cat# A8010 Histopaque-1077 Sigma Cat# 10771 Histopaque-1119 Sigma Cat# 11191 e2 Developmental Cell 60, 1–16.e1–e5, December 15, 2025 .. Dictyostelium cell culture WT cells were derived from either the AX2 or AX3 axenic strain of Dictyostelium discoideum provided by Huaqing Cai laboratory (Chinese Academy of Sciences, China). mhcA - (originally made by the Douglas N. Robinson laboratory at Johns Hopkins University) and racE - cell lines were provided by the Huaqing Cai laboratory (Institute of Biophysics Chinese Academy of Sciences, China).



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